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normal pooled adult human epidermal keratinocytes nhk (PromoCell)

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PromoCell normal pooled adult human epidermal keratinocytes nhk

Senescence induction upregulates AR activity. AR activity measured by colorimetric assay in human <t>keratinocytes</t> either treated with (A) 10 uM H 2 O 2 or Vehicle (Veh- dH 2 O) (B) 25 mM high glucose (HG) or normal glucose (5 mM) (NG) for 48 h (C) 200 nM Mitomycin-C (MMC) for 24 h compared to Veh (DMSO) and (D) 0.2 nM AT-001 for 48 h vs. Veh (1:5,000 dilution DMSO in PBS). Data are presented as the mean ± SEM. n = 4 per group obtained from two consecutive experiments. Unpaired t-test was implemented for comparison between two groups. p* < 0.05 vs. Veh or NG.

Normal Pooled Adult Human Epidermal Keratinocytes Nhk, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+pooled+adult+human+epidermal+keratinocytes+nhk/pmc11685203-27-0-10?v=PromoCell
Average 95 stars, based on 95 article reviews

normal pooled adult human epidermal keratinocytes nhk - by Bioz Stars, 2026-07

95/100 stars

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1) Product Images from "The aldose reductase inhibitors AT-001, AT-003 and AT-007 attenuate human keratinocyte senescence"

Article Title: The aldose reductase inhibitors AT-001, AT-003 and AT-007 attenuate human keratinocyte senescence

Journal: Frontiers in Aging

doi: 10.3389/fragi.2024.1466281

Senescence induction upregulates AR activity. AR activity measured by colorimetric assay in human keratinocytes either treated with (A) 10 uM H 2 O 2 or Vehicle (Veh- dH 2 O) (B) 25 mM high glucose (HG) or normal glucose (5 mM) (NG) for 48 h (C) 200 nM Mitomycin-C (MMC) for 24 h compared to Veh (DMSO) and (D) 0.2 nM AT-001 for 48 h vs. Veh (1:5,000 dilution DMSO in PBS). Data are presented as the mean ± SEM. n = 4 per group obtained from two consecutive experiments. Unpaired t-test was implemented for comparison between two groups. p* < 0.05 vs. Veh or NG.

Figure Legend Snippet: Senescence induction upregulates AR activity. AR activity measured by colorimetric assay in human keratinocytes either treated with (A) 10 uM H 2 O 2 or Vehicle (Veh- dH 2 O) (B) 25 mM high glucose (HG) or normal glucose (5 mM) (NG) for 48 h (C) 200 nM Mitomycin-C (MMC) for 24 h compared to Veh (DMSO) and (D) 0.2 nM AT-001 for 48 h vs. Veh (1:5,000 dilution DMSO in PBS). Data are presented as the mean ± SEM. n = 4 per group obtained from two consecutive experiments. Unpaired t-test was implemented for comparison between two groups. p* < 0.05 vs. Veh or NG.

Techniques Used: Activity Assay, Colorimetric Assay, Comparison

ARIs prevent HG induced senescence phenotype. Human keratinocytes were treated with 25 mM HG or normal condition medium NG. (A) SA-β-gal staining was performed to assess positive senescent cells. (B) qPCR analysis for confirming gene expression of senescent markers CDKN1A, TP53 and SERPINE1 . (C) Skin Cells stained with 5 µM DiHydroethidium (DHE) dye for 30 min to detect cytosolic superoxide and (D) cells stained with 5 µM MitoSOX dye for 10 min to detect mito superoxide. Data are presented as the mean ± SEM. n = 4 per group obtained from two consecutive experiments. Ordinary one-way ANOVA with Tukey’s multiple comparisons test was implemented for comparison between groups. p* < 0.05 vs. NG. p# < 0.05 vs. Veh.

Figure Legend Snippet: ARIs prevent HG induced senescence phenotype. Human keratinocytes were treated with 25 mM HG or normal condition medium NG. (A) SA-β-gal staining was performed to assess positive senescent cells. (B) qPCR analysis for confirming gene expression of senescent markers CDKN1A, TP53 and SERPINE1 . (C) Skin Cells stained with 5 µM DiHydroethidium (DHE) dye for 30 min to detect cytosolic superoxide and (D) cells stained with 5 µM MitoSOX dye for 10 min to detect mito superoxide. Data are presented as the mean ± SEM. n = 4 per group obtained from two consecutive experiments. Ordinary one-way ANOVA with Tukey’s multiple comparisons test was implemented for comparison between groups. p* < 0.05 vs. NG. p# < 0.05 vs. Veh.

Techniques Used: Staining, Gene Expression, Comparison

ARIs prevent H2O2 induced senescence phenotype. Human keratinocytes were treated with 10 µM H 2 O 2 or Veh (dH 2 O) and ARIs for 48 h. (A) SA-β-gal staining was performed to assess positive senescent cells. (B) Skin Cells stained with 5 µM DiHydroethidium (DHE) dye for 30 min to detect cytosolic superoxide and (C) cells stained with 5 µM MitoSOX dye for 10 min to detect mito superoxide. (D) qPCR analysis for confirming gene expression of senescent markers CDKN1A, TP53 and SERPINE1 . (E) Gamma H2A.X Staining and (F) CellEvent™ Senescence Green Detection staining in human keratinocytes treated with Veh (dH 2 O), 400 µM H 2 O 2 for 1 h followed by assessment of cells at day 10 with or without ARI AT-001. (G) ELISA to measure cytokine release in condition medium collected at day 10 and (H) qPCR analysis for confirming gene expression of senescent markers CDKN1A, TP53 and SERPINE1 CDKN2A and LMNB1 . Data are presented as the mean ± SEM. n = 4 per group obtained from two consecutive experiments. Ordinary one-way ANOVA with Tukey’s multiple comparisons test was implemented for comparison between groups. p* < 0.05 vs. H 2 O 2 . p# < 0.05 vs. Veh.

Figure Legend Snippet: ARIs prevent H2O2 induced senescence phenotype. Human keratinocytes were treated with 10 µM H 2 O 2 or Veh (dH 2 O) and ARIs for 48 h. (A) SA-β-gal staining was performed to assess positive senescent cells. (B) Skin Cells stained with 5 µM DiHydroethidium (DHE) dye for 30 min to detect cytosolic superoxide and (C) cells stained with 5 µM MitoSOX dye for 10 min to detect mito superoxide. (D) qPCR analysis for confirming gene expression of senescent markers CDKN1A, TP53 and SERPINE1 . (E) Gamma H2A.X Staining and (F) CellEvent™ Senescence Green Detection staining in human keratinocytes treated with Veh (dH 2 O), 400 µM H 2 O 2 for 1 h followed by assessment of cells at day 10 with or without ARI AT-001. (G) ELISA to measure cytokine release in condition medium collected at day 10 and (H) qPCR analysis for confirming gene expression of senescent markers CDKN1A, TP53 and SERPINE1 CDKN2A and LMNB1 . Data are presented as the mean ± SEM. n = 4 per group obtained from two consecutive experiments. Ordinary one-way ANOVA with Tukey’s multiple comparisons test was implemented for comparison between groups. p* < 0.05 vs. H 2 O 2 . p# < 0.05 vs. Veh.

Techniques Used: Staining, Gene Expression, Enzyme-linked Immunosorbent Assay, Comparison

ARIs prevent MMC induced senescence phenotype. Human keratinocytes were treated with 200 nM MMC or Veh (DMSO) and ARIs for 24 h. (A) SA-β-gal staining was performed to assess positive senescent cells. (B) qPCR analysis for confirming gene expression of senescent markers CDKN1A, TP53 and SERPINE1 . (C) Skin Cells stained with 5 µM DiHydroethidium (DHE) dye for 30 min to detect cytosolic superoxide and (D) cells stained with 5 µM MitoSOX dye for 10 min to detect mito superoxide. Data are presented as the mean ± SEM. n = 4 per group obtained from two consecutive experiments. Ordinary one-way ANOVA with Tukey’s multiple comparisons test was implemented for comparison between groups. p* < 0.05 vs. MMC. p# < 0.05 vs. Veh.

Figure Legend Snippet: ARIs prevent MMC induced senescence phenotype. Human keratinocytes were treated with 200 nM MMC or Veh (DMSO) and ARIs for 24 h. (A) SA-β-gal staining was performed to assess positive senescent cells. (B) qPCR analysis for confirming gene expression of senescent markers CDKN1A, TP53 and SERPINE1 . (C) Skin Cells stained with 5 µM DiHydroethidium (DHE) dye for 30 min to detect cytosolic superoxide and (D) cells stained with 5 µM MitoSOX dye for 10 min to detect mito superoxide. Data are presented as the mean ± SEM. n = 4 per group obtained from two consecutive experiments. Ordinary one-way ANOVA with Tukey’s multiple comparisons test was implemented for comparison between groups. p* < 0.05 vs. MMC. p# < 0.05 vs. Veh.

Techniques Used: Staining, Gene Expression, Comparison

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Journal: Frontiers in Aging

Article Title: The aldose reductase inhibitors AT-001, AT-003 and AT-007 attenuate human keratinocyte senescence

doi: 10.3389/fragi.2024.1466281

Figure Lengend Snippet: Senescence induction upregulates AR activity. AR activity measured by colorimetric assay in human keratinocytes either treated with (A) 10 uM H 2 O 2 or Vehicle (Veh- dH 2 O) (B) 25 mM high glucose (HG) or normal glucose (5 mM) (NG) for 48 h (C) 200 nM Mitomycin-C (MMC) for 24 h compared to Veh (DMSO) and (D) 0.2 nM AT-001 for 48 h vs. Veh (1:5,000 dilution DMSO in PBS). Data are presented as the mean ± SEM. n = 4 per group obtained from two consecutive experiments. Unpaired t-test was implemented for comparison between two groups. p* < 0.05 vs. Veh or NG.

Article Snippet: Normal pooled adult Human Epidermal Keratinocytes (NHK) were obtained from PromoCell (cat # C-12006) maintained and passaged in serum free Keratinocyte complete growth medium (PromoCell, cat # C-20111).

Techniques: Activity Assay, Colorimetric Assay, Comparison

Journal: Frontiers in Aging

Article Title: The aldose reductase inhibitors AT-001, AT-003 and AT-007 attenuate human keratinocyte senescence

doi: 10.3389/fragi.2024.1466281

Figure Lengend Snippet: ARIs prevent HG induced senescence phenotype. Human keratinocytes were treated with 25 mM HG or normal condition medium NG. (A) SA-β-gal staining was performed to assess positive senescent cells. (B) qPCR analysis for confirming gene expression of senescent markers CDKN1A, TP53 and SERPINE1 . (C) Skin Cells stained with 5 µM DiHydroethidium (DHE) dye for 30 min to detect cytosolic superoxide and (D) cells stained with 5 µM MitoSOX dye for 10 min to detect mito superoxide. Data are presented as the mean ± SEM. n = 4 per group obtained from two consecutive experiments. Ordinary one-way ANOVA with Tukey’s multiple comparisons test was implemented for comparison between groups. p* < 0.05 vs. NG. p# < 0.05 vs. Veh.

Techniques: Staining, Gene Expression, Comparison

Journal: Frontiers in Aging

Article Title: The aldose reductase inhibitors AT-001, AT-003 and AT-007 attenuate human keratinocyte senescence

doi: 10.3389/fragi.2024.1466281

Figure Lengend Snippet: ARIs prevent H2O2 induced senescence phenotype. Human keratinocytes were treated with 10 µM H 2 O 2 or Veh (dH 2 O) and ARIs for 48 h. (A) SA-β-gal staining was performed to assess positive senescent cells. (B) Skin Cells stained with 5 µM DiHydroethidium (DHE) dye for 30 min to detect cytosolic superoxide and (C) cells stained with 5 µM MitoSOX dye for 10 min to detect mito superoxide. (D) qPCR analysis for confirming gene expression of senescent markers CDKN1A, TP53 and SERPINE1 . (E) Gamma H2A.X Staining and (F) CellEvent™ Senescence Green Detection staining in human keratinocytes treated with Veh (dH 2 O), 400 µM H 2 O 2 for 1 h followed by assessment of cells at day 10 with or without ARI AT-001. (G) ELISA to measure cytokine release in condition medium collected at day 10 and (H) qPCR analysis for confirming gene expression of senescent markers CDKN1A, TP53 and SERPINE1 CDKN2A and LMNB1 . Data are presented as the mean ± SEM. n = 4 per group obtained from two consecutive experiments. Ordinary one-way ANOVA with Tukey’s multiple comparisons test was implemented for comparison between groups. p* < 0.05 vs. H 2 O 2 . p# < 0.05 vs. Veh.

Techniques: Staining, Gene Expression, Enzyme-linked Immunosorbent Assay, Comparison

Journal: Frontiers in Aging

Article Title: The aldose reductase inhibitors AT-001, AT-003 and AT-007 attenuate human keratinocyte senescence

doi: 10.3389/fragi.2024.1466281

Figure Lengend Snippet: ARIs prevent MMC induced senescence phenotype. Human keratinocytes were treated with 200 nM MMC or Veh (DMSO) and ARIs for 24 h. (A) SA-β-gal staining was performed to assess positive senescent cells. (B) qPCR analysis for confirming gene expression of senescent markers CDKN1A, TP53 and SERPINE1 . (C) Skin Cells stained with 5 µM DiHydroethidium (DHE) dye for 30 min to detect cytosolic superoxide and (D) cells stained with 5 µM MitoSOX dye for 10 min to detect mito superoxide. Data are presented as the mean ± SEM. n = 4 per group obtained from two consecutive experiments. Ordinary one-way ANOVA with Tukey’s multiple comparisons test was implemented for comparison between groups. p* < 0.05 vs. MMC. p# < 0.05 vs. Veh.

Techniques: Staining, Gene Expression, Comparison

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